Tag Archives: Experiment

Experiment 2- Light and Fish Health

The idea is, can light affect health of a fish?


To take on such a question, we would have to have a general ideal of how to measure health of a fish and understand what might be causing the improvement or the decline in health.

Being this back to basic chemistry 101, atoms can have negative charge, natural, or a positive charge. As these positive molecules build-up in the tank, we come to a point, where these build-ups can cause harm to the fish’s osmoregulation, which in turns affects fish’s immunity. These build-ups are oxidation and the reserve affect is reducers. Having too much oxidation can harm long term health. Having swings in oxidation and reduction can be harmful. The idea is too balance the aquarium, which is known in the aquatic industry as a balanced Redox Potential.

Anything which is added to the aquarium will affect the water electromagatism either in an up-shift of oxidation or either a downward shift in reduction. Fish detritus needs oxidation to be broken down, having too much reduction in the aquarium will not allow for decomposing of detritus. So, the best plan is to have good oxidation in the aquarium, with constant down-shifts of reduction. We don’t want to be completely oxidized or reduced.


Much more information on aquarium Redox

Redox Potential

A Healthy Aquarium-Basics

In this study, we explored if aquarium lighting acts as an oxidation or a reducer.


30gal Aquarium


(2) 12 watt GroBeam 600 LED

ORP Redox Meter


Each day, the aquarium started out in a dark room, with a starting ORP reading. This reading was noted. The aquarium lighting was switch on, which is 24 watt of TMC AquaRay 6500K XB-D GroBeam lighting. The aquarium would sit in a normal lit room with the aquarium lights on. A second reading was taken towards the end of the day, about an hour before the lights were shut off. The second reading was noted. The aquarium lights were turned off at the end of the day and the aquarium sat in a dark room all night. The readings where then retaken the next day. The experiment was ran for about a months time.



Results of light health redox acid and alklineReadingResults11


Results of lighting on aquarium


  1. The large downshift at the beginning of the readings was a difference in calibration. The readings after the 29th were much more standard with the proper calibration.
  2. The rise for oxidation are not explained.
  3. The series of days is not explained, where there was oxidation instead of the normal reduction.



There was overall a reduction affect when 6500K TMC lighting is applied to water with no other variables. While there was some days where oxidation happened, the overall affect of the lighting was a slight reduction every. Overall, the ORP reading would shift upward, then with the presence of the light, there would be a reduction. So, the line on the graph shows, a slight upward drift, with downward reductions to Redox each day.



A slight up-shift, with reductions each day due to light reduction.

Additional Discussion-

So, what can’t be explained is why on some of the days, when no variables were changed, the lighting caused a oxidation affect on the water and not reduction. There’s no explanation as to what could cause this. It could simply be a meter issue. So the reading were still noted for later exploration.

This is also our findings for 6500K lighting from one brand only.Further research could be done on specific lighting nanometers to see how different light can affect Redox. Some nanometers may cause reduction, when other causes would case oxidation. Knowing each would also to use light depending on what is happening with the tank. Like having oxidizing light, when there was an over feeding of the aquarium. These different nano meters still have to be explored.


ORP Redox Meter

TMC Aquarium LEDs


Experiment 1- Cycle Aids: Fritz vs. SeaChem

The purpose of this study is to find which aquarium cycle aid will cycle an aquarium faster and maintain more stable water parameters during the process. The idea of this experiment is establishing a healthy Nitrogen Cycle for an aquarium.

For a complete understanding of the Nitrogen Cycle, please see:

Nitrogen Cycle Basics

In-depth Nitrogen Cycle Info


API Ammonia NH3/NH4+ Test Kit

API Nitrite N02- Test Kit

API High pH Test Kit 7.4-8.8

Fritz Zyme 7 Original- Live Nitrifying Bacteria 16oz

SeaChem Stability 100mL

(3) 10 gal. glass aquarium (38L)

(3) Hydro Sponge Small(5-15gal)

(3) MA 80 Air Pump

(3) ViaAqua 50 watt heaters

(3) 5’ airline tubing

(6) Mollie Fish

(1) Spirulina fish food


Same (2) Mollie fish were placed in (3) same size aquariums (10gal). One 10gal aquarium was a control (no aid). The fish received (.5ml) of food once daily. The same weight and amount of food feed represented the same bio-load per aquarium. All aquariums had to new sponge filters ready to “seed” the aquarium. Fritz Zyme (FZ) and SeaChem Stability (SS) were used as directed (a.1) for a new aquarium “cycle” in the aquariums. Ammonia, Nitrites, and pH were check twice daily for the recommend dose time as per the directions of cycle aids. API test kits were used as directed. Results were recorded twice daily.

A high bio-load test is also preformed, by using (5mls) of fish food daily (5 days). This was to simulate poor aquarium maintenance and an increase in bio-load over a long period of time due to the maintenance. No fish were present in the aquarium. All three variables continued to be monitored.


Ammonia Readings

Nitrite Readings

pH Readings

Ammonia for Fritz SeaChem cycle aid experiement, best cycle aidNitrates for Fritz SeaChem Cycle experiment, best cycle Nitrogen CyclepH Testing


AmmoniaResults– Ammonia was the main variable measured in this experiment. SeaChem Stability was able to keep the lowest reading overall through-out the whole test. Fritz allowed for more ppm of Ammonia, but stabilize over time. The Control was able to be fairly comparable to both cycle-aids.

NitriteResults– Nitrites were the second variable of measure for toxicity of the aquarium water. Both aids were able to manage the Nitrites, while the Control had a much higher spike. During the high-bio test, Fritz did not do as well managing Nitrites.


  • SeaChem was able to keep the lowest parameters for both Ammonia and Nitrites.
  • Both aids were able to complete a healthy cycle.
  • Both aid did best controlling Nitrites, but not for a high bio test.
  • No aid or control allowed for an unmanageable reading. These levels are not best for long-term health, but are also tolerated for short periods.
  • The high bio-load test showed Fritz was not able to keep an established parameter for Nitrites. This could be because the bacteria in both cycle aids for Ammonia and Nitrites are different. The control for Nitrites for Fritz is weaker.
  • Both aids and even the Control can all be used for aiding in the Nitrogen Cycle of an aquarium. Both could also be considered for establishing a cycle. Aid and establishing are not the same.
  • pH was a secondary reading, no reading affected pH and is not really considered in this test.

Additional Discussion:

The Control was able to keep up with both aids fairly well for both aiding in the cycle of the aquarium and establishing the cycle. This is in part, because nature does a good job in managing toxic water.  The results could have also been as good as they were, because a Sponge Filter is a highly efficient filter. This experiment could have also been done using a different form of filtration, such as a Hang on the Back (HOB) or canister filter.

Fritz and SeaChem are two different approaches to live bacteria cycle aids. While both were able to aid in the cycle, but Fritz used much more aid (oz), then SeaChem. Fritz used two whole bottles (160z) for the experiment, while SeaChem used a half of a bottle (50ml total) too do the same Fritz did. The two aids also have their own smell to them, but Fritz is much stronger than SeaChem.



 Fritz Zyme:

Shake well, add directly into aquarium. Add Livestock*

New System 4oz (119ml or 1/2cup) per 10 gal (38L)

(*live stock used for bio-load of the nitrogen cycle)

SeaChem Stability:

Shake Well, Use one capful (5ml) for each 10gal (40L) on first day with a new aquarium. Then use 1 capful for each 20gal (80ml) daily for 7 days. Fish and other aquatics species may be introduced at an time as long as dosage is maintained for 7 days.

Recommend place to purchase:


The TestTest with .5ml food 20150108_103941 FritzSeaChemHigh Bio-Load Test 5ml

.5ml Food.5 ml Spirulina Food